Banca de DEFESA: LUANA CORRÊA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LUANA CORRÊA SILVA
DATE: 03/03/2022
TIME: 09:00
LOCAL: videoconferência
TITLE:

Selection based on in vitro growth characteristics of Periconia and Trichoderma endophytic fungi isolates from sugarcane roots.

KEY WORDS:

Dark Septate Endophytes; phosphate solubilization, auxin production; metal tolerance; vinasse.

PAGES: 38
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Ciência do Solo
SUMMARY:

Endophytic fungi live in the internal tissues of most plant species and are involved in the production of benefits, such as mineral nutrition, tolerance to biotic and abiotic stresses, and production of phytohormones. The objective of this work was to select isolates of endophytic fungi obtained from sugarcane roots, of the Periconia and Trichoderma genera, by in vitro growth characteristics. The experiments involved 118 strains of Periconia and Trichoderma, from sugarcane roots, from the Fungi Collection of Embrapa Agrobiologia. The experiment was in a completely randomized design (DIC) and the lines were the treatments, 6 replications, applied Scott-Knott at 5%. The strains were subcultured onto plates with malt-agar extract medium and after 4 days disks of 8 mm in diameter were removed from the edge of the colonies and inoculated in the experiments. For phosphate solubilization, Pikovskaya agar medium was used with calcium phosphate at pH 7.0 and aluminum phosphate at pH 5.0. In the Copper (Cu) tolerance experiment there were three doses of 0, 300 and 1000 mg Cu.L-¹, for Cadmium (Cd) Periconia the following doses were 0, 400 and 500 and Trichoderma 0, 200 and 300 mgCd.L and Zinc(Zn) were 0, 400 and 600 for Periconia and Trichoderma 0, 300 and 400 mg Zn.L. For vinasse, doses of 0, 400 and 800 ml.L of MEA were tested. To determine the production of acetic acid of the strains, the mycelium discs were placed in tubes containing liquid malt extract medium. All experiments were carried out with the lines incubated at 28 °C in the dark and colony growth was evaluated. Of the evaluated strains, 32 solubilized calcium phosphate, of which 65% were isolated from Periconia, where the A356 strain stood out with greater efficiency than the positive control. Twelve strains of Trichoderma were solubilized, and the A169 strain did not differ from the solubilization index of the positive control, being the most efficient in solubilization than the others. For the aluminum phosphate source, the Periconia strains did not form a halo, only the A. niger control and the Trichoderma strain A358 showed the solubilization halo. For the tolerance indexes (TI) to Cu, the Periconia A164 strains at 300 mg Cu.L-¹ very high tolerance and A163 and A164 mg Cu.L-¹ high tolerance and Trichoderma A409 and A404 at 300 mg Cu stood out. .L-¹ high tolerance and A404 in 1000 mg Cu.L-¹ moderate tolerance. The strains with the highest TI at Cd of Periconia were A423 for 400 mgCd.L-¹ and A333 and A423 for 500 mg Cd.L-¹, and the Trichoderma strains. with the highest TI were A409 and A404 for 200 mg.L-¹ and A404 for 300mg Cd.L-¹. For Periconia with higher TI to Zn were A372 and A163 for 400 and 600 mg Zn.L-¹ and Trichoderma A169 had higher TI for all dosages. The vinasse doses stimulated the growth of some Periconia and Trichoderma strains where the A316 strain had an increase of about 50% growth at the dose of 400ml.L-¹ when compared to the control and 20% at the maximum dose of 800ml.L- ¹ and the A169 strain there was an increase in growth, and in terms of indoleacetic acid production, the highlight was for A169 and A302. The strains of both the same and different species varied their aptitude for phosphate solubilization, tolerance to the tested metals, indoleacetic acid production capacity and different response to vinasse, which made it possible to select the strains with the best in vitro performance.

BANKING MEMBERS:
Externa à Instituição - ELIANE MARIA RIBEIRO DA SILVA - EMBRAPA
Interno - 927.881.579-91 - JERRI ÉDSON ZILLI - EMBRAPA
Presidente - 387406 - RICARDO LUIZ LOURO BERBARA