Banca de DEFESA: SIRAJ MOHAMMAD

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : SIRAJ MOHAMMAD
DATE: 05/02/2024
TIME: 09:00
LOCAL: Remoto
TITLE:

THE PRODUCTION OF BIOACTIVE PEPTIDES BY OPTIMIZATION THE PROCESS OF ENZYMATIC HYDROLYSIS OF PROTEIN OBTAINED FROM TILAPIA FISH SKIN (Oreochromis niloticus, LINNAEUS 1758) AND PURIFICATION THE PEPTIDES USING MACROPOROUS RESIN

KEY WORDS:

antioxidant, isotherm test, purification, adsorption, polymer, bioactive peptide, hydrolysates, collagen.

PAGES: 38
BIG AREA: Ciências Agrárias
AREA: Ciência e Tecnologia de Alimentos
SUBÁREA: Ciência de Alimentos
SUMMARY:

Enzymatic hydrolysis (EH) of proteins relies, essentially, in the enhancing of the functional and nutritional properties of proteins, such as antioxidant activity, solubility, oil holding capacity and water holding capacity, emulsification, foaming properties and sensory properties. In this study, the protein hydrolysates obtained of tilapia fish skin waste (PHTFSW) was optimized using alcalase 2.4.L by central composite design (CCD). Kinetics of the reaction was considered in relation to initial substrate concentration, initial enzyme concentration, and hydrolysis time. Protein hydrolysates were separated using three macroporous resin. Degree of hydrolysis (DH), radical scavenging activities (DPPH) and ferric reducing antioxidant power (FRAP) were used as dependent variables. temperature, pH and proportion enzyme to substrate (PE%) as independent variables.. The optimum degree of hydrolysis DH%, DPPH and FRAP were achieved at temperature 58.4 ℃, pH of 8.7 unless DPPH which was at pH of 7.0. Kinetic model presented that DH increased with increasing the initial enzyme concentration E0, under a constant substrate concentration as the hydrolysis time prolonged to 120 min. Furthermore the DH decreased with increasing the initial substrate concentration S0 under a constant enzyme concentration as the hydrolysis time prolonged to 120 min. However beyond 120 min, the DH of these samples did not vary significantly. XAD-7HP showed the highest adsorption and desorption capacities. The isotherm tests indicated that the adsorption mechanism was better explained using Freundlich model which provided great accuracy in the separation of PHTFSW for XAD-7HP resin. Purified and normal extract of PHTFSW showed high antioxidant capacity in both methods of FRAP and DPPH. This purified PHTFSW could potentially find application as raw natural bioactive compounds in the cosmetic and pharmaceutical industry preparations.

COMMITTEE MEMBERS:
Externa à Instituição - ALINE SOARES CASCAES TELES - INT
Interna - 3333721 - GESILENE MENDONCA DE OLIVEIRA
Presidente - 2455049 - JOSE LUCENA BARBOSA JUNIOR
Externo à Instituição - MARCUS VINICIUS DA SILVA FERREIRA
Externa à Instituição - RENATA OLIVEIRA SANTOS