Evaluation of the Concentration by Nanofiltration of Blackberry Juice on the Composition and Bioaccessibility of Anthocyanins
Filtration process, Bioactive compounds, Antioxidant properties, Red fruits
The consumption of fruit juices rich in bioactive molecules is important primarily due to the health benefits these compounds can offer. Blackberries (Rubus spp.), cultivated in Brazil, particularly in the southern and southeastern regions, are known for their high content of bioactive compounds like anthocyanins and phenolic compounds. These compounds have antioxidant and anti-inflammatory properties, helping to reduce the risk
of cardiovascular, metabolic, and neurodegenerative diseases. This study aimed to concentrate anthocyanins from blackberry pulp using the nanofiltration process to enhance the nutritional and functional value of products derived from this fruit. Three different nanofiltration membrane modules were evaluated: tubular, spiral, and plate-and- frame geometries. Results indicated that the tubular and plate-and-frame membranes were inefficient at concentrating the bioactive compounds, resulting in a low anthocyanins yield. Conversely, the spiral membrane was more effective, with a concentration factor (CF) of 2.91. In this configuration, anthocyanins concentration increased by 47%, total phenolic content by 127.47%, and antioxidant activity by 102.86% in the concentrated product. Following the selection the membrane, the concentrated product will be evaluated for the stability of anthocyanin concentration and color under different storage conditions, including temperature variations, light exposure, and pH levels. This concentrated product will be used as an ingredient in the mixed juice formulation based on apple juice. The bioaccessibility of the bioactive compounds present in the mixed juice will be evaluated to determine how much of these compounds remain accessible for absorption by the body. The in vitro digestion assay will be conducted according to the Infogest protocol. This work is expected to contribute to demonstrating the efficacy of the health benefits provided by the consumption of products rich in anthocyanins and other essential nutrients.