Banca de DEFESA: SIRAJ MOHAMMAD

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : SIRAJ MOHAMMAD
DATA : 02/08/2019
HORA: 09:00
LOCAL: Sala de Defesas do PPGCTA
TÍTULO:

Separation and purification of anthocyanin compounds from grape wine pele pomace (Alicante Bouschet) using macroporous resins.

PALAVRAS-CHAVES:

purification, polyphenol, extraction, antioxidant

PÁGINAS: 104
GRANDE ÁREA: Ciências Agrárias
ÁREA: Ciência e Tecnologia de Alimentos
SUBÁREA: Tecnologia de Alimentos
ESPECIALIDADE: Aproveitamento de Subprodutos
RESUMO:

Grapes are one of the most commonly produced fruit crops in the world and almost 65% of their production is used to make wine, grapes and juice. Consequently, the grape industry produces large quantities of grape pomace which is a rich source of health promoting polyphenols, such as proanthocyanidins, flavones and flavan-3-ols such as flavonoids (quercetin, kaemferol, myricetin), catechins or phenols (carnosol, rosmanol, rosamaridifenol) and phenolic acids (carnosic acid, rosmarinic acid). The antioxidant capacity of certain substances has also been of interest since they help the body to protect itself against damage caused by reactive oxygen species (ROS), reactive nitrogen especies (RNS) and reactive chlorine especies (RCS), associated with degenerative diseases. Therefore; in this study, the raw material used was the residue from grape fermentation of Alicante Bouschet from the production of red wine, (1 g) of dry GP was weighed and extracted with 12.5 ml of 50% water and 50% ethanol, the residue was extracted again by the same volume of solution, the adsorption/desorption characteristics of grape wine peel pomace (GWPP) anthocyanins (MA) on six types of macroporous resins (XAD 2, XAD 4, XAD 7HP, XAD 8, XAD 11 and XAD 16 were investigated. XAD-7HP and DAX 8 showed higher adsorption/desorption capacities. On basis of static adsorption test, the adsorption mechanism indicated that the process was better
explained by pseudo-first-order kinetics and the Langmuir isotherm model. Adsorption isotherm tests showed that the adsorption process was exothermic on XAD 7HP and DAX 8 when performed at 30° C, 40 °C and 50 °C. The results of antioxidant tests indicate that purified anthocyanin extract showed high antioxidant capacity in the both methods of FRAP and DPPH Regarding to high-performance liquid chromatography analysis, acylated Peonidin, Peonidin-3-glucoside, acylated malvidin and malvidine-3-glucoside are the four main anthocyanin compounds in the initial and purified extract of GWPP. Overall, macroporous resins can be an effective tool to purificate anthocyanin instead of using organic solvents and supply pharmaceutical and food industry with as safe and cheap process.

MEMBROS DA BANCA:
Presidente - 2455049 - JOSE LUCENA BARBOSA JUNIOR
Externo à Instituição - JUAREZ VICENTE - UFRRJ
Externo à Instituição - NATHÁLIA DA ROCHA RODRIGUES - UES