Banca de DEFESA: NATHÁLIA LEAL MENDES
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.DISCENTE : NATHÁLIA LEAL MENDES
DATA : 20/06/2018
HORA: 08:30
LOCAL: UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO
TÍTULO:
Study of the Jamelão fruit peel (Syzygium Cumini (L.) Skeels) by CLAE-DAD and CLUE-QTOF-EM-EM.
PALAVRAS-CHAVES:
anthocyanins, flavonoids, HPLC-DAD, UPLC-EM-EM
PÁGINAS: 66
GRANDE ÁREA: Ciências Agrárias
ÁREA: Ciência e Tecnologia de Alimentos
RESUMO:
The jamelão (Syzygium cumini (L) Skeels.) fruit properties are generally related to the antioxidant activity of the phenolic compounds present in its composition. The objective of this dissertation was to extract, isolate and identify anthocyanins present in the jamelão fruit peel (Syzygium cumini (L.) Skeels) by HPLC-DAD and identification of flavonoids by UPLC-EM-EM. After the freeze-drying of the fruit peels, the extracts were prepared (non- anthocyanic phenolic fraction, crude extract (I) and anthocyanin extract (II)). For the non-anthocyanic phenolic fraction, a solution of 60% acetone in water, HPLC-DAD isolation and identification of non-anthocyanic phenolics by UPLC-EM-EM was used. For the crude extract a solution of 10% MeOH of formic acid was used. For the anthocyanic extract: extraction with 70% acetone in Milli-Q water, isolation with Oasis® MCX Cartridge Waters cartridge and identification by HPLC-DAD. Measure the antioxidant capacity by ABTS and ORAC, quantification of total phenolic compounds of extracts I and II of the years 2017 and 2018. And evaluation of the stability of anthocyanins extracts I and II of the powder of the year 2017. Were identified by UPLC-EM-EM to myricetin-3-O-galactoside (TR = 5.27), myricetin-3-O-glycoside (TR = 5.49), mirecitin-3-O-pentose (TR = 6) (TR = 7.74), quercitin-3-O-hexose (TR = 8.04), laricitrin-3-O-glucuronide (TR = 7.84), siringetin -3-O-galactoside (TR = 10.33), sirigetin-3-O-glycoside (TR = 10.56), laricitrin-3-O-glucuronide and quercitin-3-O-hexose were identified for the first time in the fruit of the swineherd. The anthocyanins identified were: delphinidin-3,5-O-diglycosylated (TR = 2.8), cyanidin-3,5-O-diglycosylated (TR = 3.9), petunidine-3,5-O-diglycosylated (TR = 4.6), malvinidine-3,5-O-diglycosylated (TR = 6.9), petunidine-3,5-O-diglycosylated being the major anthocyanin. For analysis of total phenolics for extract I the average found for the batch of 2017 was 4740.32 mg.EAG.100g-1 and for the batch of 2018 it was 8351.87 mg.EAG.100g-1. For extract II of the 2017 batch, the mean was 469.18 mg.EAG.100g-1 and the 2018 batch was 482.47 mg.EAG.100g -1. For the antioxidant capacity by ABTS, extract I year 2017 (178.78 μM.Trolox.g-1) and 2018 (739.30 μM.Trolox.g-1) and extract II year 2017 (32.17 μM. Trolox.g-1) and 2018 (36.89 μM.Trolox.g-1) for ORAC, extract I year 2017 (499.91 μM.Trolox.g-1) and 2018 (759.33 μM.Trolox. g-1) and extract II year 2017 (147.51 μM.Trolox.g-1) and 2018 (169.56 μM.Trolox.g-1). And the stability of the total anthocyanins of extract I in the period from 0 to 60 days did not vary significantly (day (0) 4.24 mg.100g-1 and sixty (60) 4.50 mg.100g-1), extract II day (0) 2.27 mg.100g-1 and sixty (60) 1.69 mg.100g-1). E monomer extract I in the 0-60 day period did not significantly change (day (0) 3.96 mg.100g-1 and sixty (60) 4.34 mg.100g-1), extract II (day (0) 2. 20 mg.100g-1 and sixty (60) 1.62 mg.100g-1). Jamelão showed to be a source of phenolic compounds (anthocyanins and flavonoids) with important nutritional factor and potential of cultivation in Brazil by fruits such as jamelão considered a non - commercial fruit.
MEMBROS DA BANCA:
Externo ao Programa - 1488978 - CRISTIANE HESS DE AZEVEDO MELEIRO
Externo à Instituição - FLAVIA DOS SANTOS GOMES - EMBRAPA
Interno - 1524368 - MARIA IVONE MARTINS JACINTHO BARBOSA