Banca de DEFESA: MATHEUS RODRIGUES SILVA DO CARMO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MATHEUS RODRIGUES SILVA DO CARMO
DATE: 16/06/2020
TIME: 14:00
LOCAL: Videoconferência RNP
TITLE:

SELECTION OF POTENTIALLY PROBIOTIC LACTOBACILLUS BASED ON PATHOGENS INHIBITION CAPACITY AND ADHESION IN MUCOSA

KEY WORDS:

lactobacillus, probiotics, adhesion, mucous, microbiota, ht-29

PAGES: 27
BIG AREA: Ciências Agrárias
AREA: Ciência e Tecnologia de Alimentos
SUBÁREA: Ciência de Alimentos
SPECIALTY: Microbiologia de Alimentos
SUMMARY:

Manystudieshaveshowntheimportanceofunderstandingthecomplexityofthe intestinal microbiota andtherelationtohumanhealth. The homeostasisofthe microbiota maydependonnumerousfactorssuch as regular intakeoffoodsenrichedwithprobiotics. Moreover, imbalancecan lead totheconditioncalleddysbiosis, a major publichealthconcern. Probiotics are microorganismsthat, wheningested in adequateamounts, conferbenefitstothe host health. In order for bacteriatobeconsideredprobiotic, they must meetcertainrequirementssuch as intestinal mucosaladhesioncapacityandinhibitionofpathogens. Lactobacillus are partofthegroupoflacticacidbacteria (LAB)withimportanttechnologicalandhealthproperties. The objectiveofthestudyistoevaluatetheadhesionpropertiesofLactobacillusisolatedfromhuman, withrespecttotheadhesion, colonizationandinhibitioncapacityofpotentialpathogens. The followingstrainsofLactobacilluswillbeused: 4 strainsof L. paracasei, 3 strainsof L. rhamnosusand 2 strainsof L. fermentum. Pathogeninhibitionwillbeevaluatedbyreducingtheproductionofbiofilmsandin vitro inhibitionofpathogens, theidentificationoftheassociatedLactobacillus genes in theadhesionin mucosa andtheadhesioncapacityofLactobacillus spp. in lineofmucus-producingcells (HT-29). Finally, thehydrophobicitytestofthecellsurfaceofLactobacilluswillbeperformed as oneoftheparameterstoevaluatetheadhesioncharacteristicofthesemicroorganisms in intestinal mucosa.Withthedevelopmentofthiswork, it isexpectedtoselectstrainsofisolated lactobacilli fromhumanthat shows a betterprobiotic profile basedonthecapacityofadhesion in mucosa.

BANKING MEMBERS:
Presidente - 359403 - ROSA HELENA LUCHESE
Interna - 1806986 - ELISA HELENA DA ROCHA FERREIRA
Externo à Instituição - ANDRE FIORAVANTE GUERRA - CEFET/RJ