KINETIC MODEL FOR OPTIMIZED CONDITIONS OF PROTEIN HYDROLYSIS OBTAINED FROM TILAPIA FISH SKIN WASTE (Oreochromis niloticus, Linnaeus 1758) USING ALCALASE 2.4.L AND PURIFICATION OF PROTEIN HYDROLYSATES USING MACROPOROUS RESIN
biological peptides, separation, adsorption, enzyme.
This work focus on protein hydrolysis due to the important role enzyme-modified hydrolysates, including their functional properties and their usefulness as intermediate ingredients in the cosmetics, pharmaceutical, nutraceutical and food sectors. This process generates a large amounts of bioactive peptides with different properties and applications, requiring the application of separation techniques to purificate these compounds. Conventional methods for separating protein hydrolysates, such as membrane separation, ultrafiltration and gel filtration, and liquid-liquid extraction, present problems associated with low purity, low yield of target peptides and low conversion rate of the substrate protein. The adsorption macroporous resins are often applied in the isolation and purification of natural products such as polyphenols, flavonoids, antioxidants, alkaloids and amino acids. Furthermore, there are no reports of the best conditions of the enzymatic hydrolysis process to obtain protein hydrolysates from tilapia fish skin residues using macroporous adsorption resins. The objective of this study is to identify the best conditions for enzymatic hydrolysis of proteins from skin residues of tilapia fish using the enzyme alkalase to obtain a higher degree of protein hydrolysis of and to identify the best kind of resin to separate and purify protein hydrolysates from tilapia fish skin wastes (PHTFSW).