Banca de DEFESA: MARIA EDUARDA DOS SANTOS LOPES FERNANDES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARIA EDUARDA DOS SANTOS LOPES FERNANDES
DATE: 07/12/2023
TIME: 09:00
LOCAL: Google meet
TITLE:

Influence of free omental graft without microvascular anastomosis on the integration of mesh skin grafts applied in experimental wounds in rabbits.

KEY WORDS:

Omentum; Oryctolagus cuniculus; Reconstructive Surgery; Wound Healing

PAGES: 113
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Cirúrgica Animal
SUMMARY:

Faced with the challenges in the use of autologous skin grafts in veterinary surgery, the search for alternatives to enrich the repair process is progressively growing. The present work aims to evaluate the influence of the omental graft without vascular microanastomosis (WVMA) on the implantation of mesh skin grafts applied to wounds experimentally produced in rabbits. We used 24 rabbits that underwent median longitudinal celiotomy to collect an omental fragment (4 cm²). After celiorrhaphy, two 4 cm² square wounds were produced in all animals, one on each side of the dorsal thoracic region, so that each segment of skin removed was prepared to serve as a mesh skin graft, applied to the contralateral wound. In control wounds (CW), the skin graft was sutured to the recipient bed using separate single sutures with 4-0 polyamide suture, without the presence of the omentum fragment between the graft and the recipient bed. In the treated wounds (OW), the omentum fragment was implanted in the recipient bed using eight separate simple sutures with 4-0 polydioxanone thread at its ends, with subsequent suturing of the skin graft as previously described. Postoperatively, antibiotic, and analgesic therapy, a tie over dressing on the wounds using water-soluble lubricating gel, changed approximately every 3 days, as well as a protective collar and taped boots to prevent self-mutilation, were instituted. The wounds were evaluated for clinical appearance on postoperative days 3, 7, 10, 14, 17, 21 and 28, assigning scores to the characteristics: increase in volume, color, exudate, devitalization, and suture dehiscence. Furthermore, the animals were kept for periods of 7, 14 and 28 days (eight animals in each period), when they were then euthanized for macroscopic anatomopathological evaluation (post-mortem) of the wounds and obtaining material for histopathology (microscopic evaluation). Clinically, the increase in volume in the OW stood out, which remained longer and more intense, especially with a firm consistency, on the side of the grafts from the third day after surgery. Furthermore, the production of inflammatory exudate for a longer period was also observed in the OW, with a faster evolution of the grafts to dark colors with a dry and devitalized appearance throughout the evaluation moments, with no integration of the skin graft in any animal in this group. The macroscopic anatomopathological evaluation showed a more intense inflammatory reaction in the OW at all evaluation moments, but with a slowdown in these reactions and progression to tissue repair over time in both groups. Microscopy corroborated the data from the other evaluations, making it possible to observe more inflammation, with a greater quantity of polymorphonuclear, mononuclear and giant cells on days 14 and 28 post-operatively, greater intensity of ulcers in the epidermis on the twenty-eighth day and less re-epithelialization in the days 14 and 28 in OW compared to CW. It is concluded that the LSMAV omental graft, despite having remained viable and without deleterious effects on the application site (recipient bed), did not exert a positive influence on the integration of mesh skin grafts applied to experimental rabbit wounds.

COMMITTEE MEMBERS:
Interno - 3015985 - FELIPE FARIAS PEREIRA DA CAMARA BARROS
Interna - 3247698 - TICIANA DO NASCIMENTO FRANCA
Externo à Instituição - ANDRIGO BARBOZA DE NARDI
Externa à Instituição - PAULA VELOZO LEAL
Externo à Instituição - TIAGO DA CUNHA PEIXOTO - UFBA
Externo à Instituição - NEY LUIS PIPPI
Externa à Instituição - MARTA FERNANDA ALBUQUERQUE DA SILVA