Banca de DEFESA: KARLA GEOVANNA PEREIRA NASCIMENTO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : KARLA GEOVANNA PEREIRA NASCIMENTO
DATE: 08/03/2024
TIME: 15:00
LOCAL: Instituto de Veterinária - Online
TITLE:

Immunohistochemical evaluation of CD123+ cells in lymph nodes of animal species

KEY WORDS:

CD123, plasmacytoid dendritic cells, pathology

PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SPECIALTY: Anatomia Patologia Animal
SUMMARY:

Plasmacytoid dendritic cells (pDCs) represent a subgroup of dendritic cells present in peripheral blood and lymphoid organs. These cells are identified by the high expression of the interleukin-3 α chain receptor (IL-3Rα), and produce large amounts of interferon type-I (IFN-I), mediating the immune response. In immunohistochemistry, the marker used for the detection of this cell type is the anti-CD123 antibody. Plasmacytoid dendritic cells have become the target of studies in human medicine for the diagnosis and development of antitumor therapies for neoplasms of hematological origin, inflammatory processes and autoimmune diseases, and in veterinary medicine, little is known about the existence of plasmacytoid dendritic cells and their distribution in normal lymphoid tissue. In dogs, there is only one study that used the anti-CD123 antibody for immunostaining of pDCs in mammary tumors of, associating its presence with a worse tumor prognosis. Thus, this study aims to verify if this marker is efficient for the detection of CD123+ cells in lymph nodes of dogs, cats, cattle and horses and, if in these species, the distribution is similar to that observed in humans. Immunohistochemistry with monoclonal and polyclonal anti-CD123 antibody was performed in 10 samples of bovine, feline and canine species, and 5 samples of equine species. The results obtained with monoclonal antibody were not satisfactory in the lymph nodes of the animals. On the other hand, with polyclonal antibodies, CD123+ cells were found to be grouped in clusters or individualized predominantly in the paracortex of practically all canine (10/10) and bovine (8/10) samples. In felines, the results were negative in all lymph nodes, and in horses, the results were disregarded due to the presence of hemosiderin. It was concluded that CD123 is a valid marker for use in animals, and that these cells, as well as in humans, are more frequently distributed in the paracortical region of the lymph node.

COMMITTEE MEMBERS:
Presidente - 3247698 - TICIANA DO NASCIMENTO FRANCA
Interna - 2606155 - VIVIAN DE ASSUNCAO NOGUEIRA CARVALHO
Externa ao Programa - 3377607 - APARECIDA ALVES DO NASCIMENTO - UFRRJExterna à Instituição - CLARICE MACHADO DOS SANTOS - UFF
Externa à Instituição - VALIRIA CERQUEIRA DUARTE - UFPA