Banca de DEFESA: YASMIM SANTOS KAULICH DE SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : YASMIM SANTOS KAULICH DE SOUZA
DATE: 10/02/2026
TIME: 10:00
LOCAL: Google meet
TITLE:

Evaluation of the antinociceptive and anti-inflammatory potential of Trema micrantha (L.) Blume. (Cannabaceae) extract in mice

KEY WORDS:

Endocannabinoid system, Pain, cannabimimetics, inflammation

PAGES: 129
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Farmacologia e Terapêutica Animal
SUMMARY:

Ethnobotanical research into the pharmacological potential of traditionally used plants is important for the development of alternatives to drugs commonly associated with undesirable side effects. Furthermore, global public health issues linked to opioid abuse and clinical limitations related to the use of non-steroidal anti-inflammatory drugs and corticosteroids motivate this work. Trema micrantha (Cannabaceae) is a species widely distributed in Brazil, used in folk medicine for skin rashes, antirheumatic and antimicrobial action. Recently, it has been described as secreting phytocannabinoids in low concentrations compared to Cannabis sativa, although its actual mechanisms of action and pharmacological profile have not been fully elucidated. This study aimed to evaluate the antinociceptive and anti-inflammatory potential of hydroethanolic extracts of leaves and stem of trema micrantha in experimental models of acute pain, neuropathic pain, and inflammation in mice. In the acetic acid-induced abdominal contortion model, only the highest dose of the leaf extract showed a response, while with the stem extract, the two highest doses tested showed an effect. The increase in latency time in the tail withdrawal model with the compound was achieved by all extracts and appeared to have a dose-dependent behavior. We investigated the possible pathways involved in the mechanism of action of the compounds through the prior administration of antagonists in the same model. The leaf extract (100 mg/kg) did not demonstrate involvement with muscarinic receptors, µ and δ opioid receptors; other pathways such as nitrergic, ATP-dependent potassium channel, endocannabinoid, and κ opioid receptors appear to be involved in the mechanism of action, since antagonists of these inhibited the effect of the extract. In contrast, the stem group (100 mg/kg) suggested greater involvement, being inhibited even by atropine, nor-binalorphimine, and methylnatrexone. In the Von Frey model, we evaluated allodynia 15 days after sciatic nerve constriction. Only the stem extracts demonstrated dose-dependent antinociceptive activity. In the rotarod model, where we verified the compound's influence on animal mobility, there was no interference in motor performance in any group. Anti-inflammatory activity was evaluated in carrageenan-induced inflammation models in the paw edema model. Doses of 50 and 100 mg/kg of both extracts were able to reduce edema in a dose-dependent manner. Furthermore, when induced by histamine, the same doses reduced edema, but the stem extract also showed an effect at a dose of 10 mg/kg, inhibiting histamine action. In the subcutaneous air pouch model, we observed that leukocyte migration was reduced, as was the dosage of inflammatory mediators (TNF-α, IL-1β, and IL-6), by both extracts; however, the stem extract at a dose of 100 mg/kg did not inhibit leukocyte recruitment or IL-1β production. In the acute toxicological assay at a dose of 1 g/kg, 3/6 animals died within 24 hours. We can suggest that T. micrantha extracts may have some interaction with the aforementioned pathways, characterizing their demonstrated antinociceptive and anti-inflammatory activity. A subsequent phytochemical characterization between leaves and stem is important in elucidating the substances responsible for the results found. We suggest that the interaction of phytocannabinoids and other phyto compounds of the plant may be related to the regulation of the tested pathways. In addition, a targeted toxicological study is an important step in advancing its therapeutic use.

COMMITTEE MEMBERS:
Presidente - 1674073 - BRUNO GUIMARAES MARINHO
Externa ao Programa - 2479104 - NORMA APARECIDA ALMEIDA FIGUEIREDO DE OLIVEIRA - UFRRJExterno ao Programa - 386960 - WELLINGTON DA SILVA CORTES - UFRRJExterno à Instituição - STELIO PACCA - UNESP
Externo à Instituição - CARLOS GIOVANI DE OLIVEIRA NASCIMENTO - UNIFAL-MG