Banca de QUALIFICAÇÃO: ALINE TONUSSI DA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ALINE TONUSSI DA SILVA
DATA : 28/11/2019
HORA: 10:00
LOCAL: Salão Verde
TÍTULO:

Hematology, serum biochemistry and clinical evaluation associated with molecular and serological detection of Leishmania spp. in dogs from shelters in the metropolitan region of Rio de Janeiro. 

PALAVRAS-CHAVES:

Domestic dogs; Leishmaniasis; Shelters

PÁGINAS: 79
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
RESUMO:

Leishmaniasis is a disease caused by protozoa of the genus Leishmania and transmitted by hematophagous sandfly vectors of the genus Lutzomyia that infect a wide variety of mammalian hosts, including dogs and humans. Being considered a reemerging anthropozoonosis and a major public health problem, it represents a complex of diseases with important clinical spectrum and epidemiological diversity. The importance of the canine reservoir derives from the close and frequent contact between dogs and humans, and the fact that animals may present asymptomatic infection despite a high degree of parasitism on healthy skin and viscera. The aim of the present study was to evaluate hematological, biochemical and clinical changes in dogs positive for Leishmania spp. by serological and molecular methods. Blood samples from 166 shelters dogs in the metropolitan region of Rio de Janeiro were collected. Hematological and serum biochemical analysis of the animals, as well as the detection of antibodies anti-Leishmania spp. by double-path immunochromatographic rapid test TR DDP - (Fiocruz®) and Indirect Immunofluorescence Reaction (RIFI) were performed. In addition, serologically positive animals were molecularly evaluated by polymerase chain reaction (PCR). The results showed that (18/166) 10,84% of the dogs presented seroreactivity by TR DDP, and these samples also underwent IFAT, in which 100% (18/18) of the animals were seroreagent. In PCR, based on the portion of the v7-v8 18S SSU rRNA gene, 16,66% (3/18) of shelter dogs were positive. No hematological, biochemical and clinical alterations were observed with the infection. The results show the importance of monitoring canine leishmaniasis, especially in shelter dogs so that control and prevention strategies can be implemented properly, minimizing the deleterious effects of this zoonosis.

MEMBROS DA BANCA:
Externo ao Programa - 386994 - CARLOS HENRIQUE MACHADO
Interno - 2318733 - DANIEL DE ALMEIDA BALTHAZAR
Presidente - 2606155 - VIVIAN DE ASSUNCAO NOGUEIRA CARVALHO