Banca de DEFESA: NATÁLIA D' ASSUMPÇÃO LIMA RANGEL

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : NATÁLIA D' ASSUMPÇÃO LIMA RANGEL
DATE: 21/02/2022
TIME: 14:00
LOCAL: Google meet
TITLE:

Evaluation of the effect of maternal consumption of a high-fat diet on cardiac function in the profile of rats.

KEY WORDS:

Metabolic programming, heart failure, angiotensin II, AT2 receptor.

PAGES: 107
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Clínica e Cirurgia Animal
SPECIALTY: Clínica Veterinária
SUMMARY:

Imbalanced nutrition is one of the risk factors for obesity and metabolic syndrome. Overweight pregnant women, resulting from inadequate eating habits, can trigger metabolic and cardiovascular disorders in their offspring. Previously, our laboratory demonstrated that maternal consumption of a high-fat diet, before and during pregnancy and lactation, promoted greater body mass, adiposity, hyperleptinemy, left ventricular hypertrophy and changes in the gene expression of hypertrophy and heart failure (HF) markers in offspring at weaning. In addition to systolic dysfunction at 30 days of age. Cardiac changes was observed with an increase in the protein content of the AT1 receptor for angiotensin II (AngII) only in females from offspring at weaning. The renin angiotensin system (RAS) heart expression seems to promote pro- and hypertrophic process and sympathoexcitatory effect in HF. This study aimed to investigate whether maternal consumption of a high-fat diet promotes HF, and to correlate this phenotype with changes in the RAS and the local sympatho-adrenergic system in adult offspring. Wistar rats received a control diet (9% lipids) or high fat (29% lipids) for 8 weeks before mating, during pregnancy and lactation. At 21 days of age, offspring were weaned and separated into control or high fat offspring. Both offspring received only control diet from weaning to 180 days of age. The body weight offsprings gain was monitored from birth; and the food intake was analyzed from the prepubertal period to 180 days of age. The cardiac function of the offspring at 180 days of age was evaluated by echocardiography and by the isolated heart technique (Langendorff) after treatment with isoproterenol in increasing doses. The animals were euthanized by the guillotine method, and the heart and white adipose tissue (retroperitoneal, inguinal and Perigonadal) were weighed. The left ventricular posterior wall was used to evaluate the protein expression of RAS components (renin, angiotensinogen, ACE1) and of AT1 and AT2 receptors for AngII and β1-adrenergic, using the Western Blotting technique. Females and males of the DH offspring showed systolic dysfunction (reduction in ejection fraction and systolic output) at 180 days of age, with no changes in body weight or adiposity. The males of the high fat offspring in adulthood showed structural changes in the heart and greater cardiac responsiveness to adrenergic stimulation. AT2 protein expression was higher in adult females of high fat offspring, and reduced in males. These results suggest that maternal consumption of a high fat diet promotes systolic dysfunction in females and males of adult offspring; however, males have a compensatory mechanism through the adrenergic system. Furthermore, we suggest that the change in AT2 receptor protein expression in females is a protective effect, while the reduction of this receptor in adult male offspring is involved with the cardiac remodeling process.

BANKING MEMBERS:
Interno - 4563410 - JULIO ISRAEL FERNANDES
Externo ao Programa - 2225596 - EMERSON LOPES OLIVARES
Externa ao Programa - 2479104 - NORMA APARECIDA ALMEIDA FIGUEIREDO DE OLIVEIRA
Externo à Instituição - ANDRE DE SOUZA MECAWI - UNIFESP
Externo à Instituição - DANIEL BADAUE PASSOS JUNIOR - UFS